Identification and Characterization of BenzimidazoleResistance in Monilinia fructicola from Stone Fruit OrchardsinCalifornia
نویسندگان
چکیده
منابع مشابه
Factors Affecting Latent Infection of Prune Fruit by Monilinia fructicola.
ABSTRACT Experiments were conducted in three prune orchards in California. In each orchard, inoculations with Monilinia fructicola, the causal agent of brown rot of stone fruits, were performed on branches of trees at bloom and fruit developmental stages. Five inoculum concentrations were used in each inoculation. Six and four wetness durations were created for each inoculum concentration at bl...
متن کاملFungicide-induced transposon movement in Monilinia fructicola.
Repeated applications of fungicides with a single mode of action are believed to select for pre-existing resistant strains in a pathogen population, while the impact of sub-lethal doses of such fungicides on sensitive members of the population is unknown. In this study, in vitro evidence is presented that continuous exposure of Monilinia fructicola mycelium to some fungicides can induce genetic...
متن کاملMolecular cloning, characterization, and expression of a redox-responsive cutinase from Monilinia fructicola (Wint.) Honey.
cDNA clones encoding a cutinase expressed in cutin-induced cultures of the plant pathogen Monilinia fructicola were isolated using a protein-based strategy. The largest cDNA (Mfcut1) was found to contain an open reading frame of 603 bp that predicted a 20.2-kDa protein of 201 amino acids with a 20-amino-acid secretory signal peptide and a pI of 8.4. The predicted protein contained cutinase/lipa...
متن کاملAffinity purification and characterization of a cutinase from the fungal plant pathogen Monilinia fructicola (Wint.) honey.
Trifluoromethyl ketones (TFK) are potent inhibitors of a variety of serine hydrolases. The TFK inhibitor, 3-(4-mercaptobutylthio)-1,1,1-trifluoro-2-propanone (MBTFP), was found to competitively inhibit cutinase activity (I50 = 9.4 x 10(-3)) from the fungal plant pathogen Monilinia fructicola and to serve as an effective affinity ligand for the purification of cutinases from culture filtrates. T...
متن کاملIdentification of Monilinia fructigena, M. fructicola, M. laxa, and Monilia polystroma on Inoculated and Naturally Infected Fruit Using Multiplex PCR
Marie-José Côté, Canadian Food Inspection Agency, Ottawa Laboratory (Fallowfield), Centre for Plant Quarantine Pests, 3851 Fallowfield Road, Ottawa, Ontario, Canada, K2H 8P9; Marie-Claude Tardif, Health Canada, Food Directorate, Building #7, Tunney’s Pasture, P.L. 0700E1, Ottawa, Ontario, Canada K1A 0L2; and Allison J. Meldrum, Canadian Food Inspection Agency, Ottawa Laboratory (Fallowfield), C...
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ژورنال
عنوان ژورنال: Applied and Environmental Microbiology
سال: 2003
ISSN: 0099-2240,1098-5336
DOI: 10.1128/aem.69.12.7145-7152.2003